自然》，第601卷，第600-605页。

　　类人胚胎体模拟人类早期胚胎发育和人类胚胎植入子宫的情况

　　Harunobu Kagawa等人 奥地利科学院分子生物技术研究所

　　遵义供卵试管助孕哪家机构号

　　2、受精后一周内，人类胚胎形成囊胚并在子宫内筑巢。建立人类囊胚的模型对人类的生育能力具有重要意义。然而，要建立一个能够准确模拟和对应囊胚不同细胞类型的发育顺序并再现囊胚形态的模型仍然非常困难。

　　该团队通过抑制三种信号通路：Hippo、TGF-和ERK，成功地利用人类多能干细胞构建了类似于人类囊胚的结构。

　　该研究团队通过在人类多能干细胞中三重抑制Hippo、TGF- 和ERK三个信号通路形成胚泡，成功诱导产生了包含三个原始细胞谱系的囊胚期类似物。另外，该团队观察到外胚层诱导极性滋养外胚层的局部成熟，从而赋予囊胚定向附着在激素刺激的子宫内膜细胞上的能力。

　　a,Schematic representation of the time window of human peri-implantation development modeled by blastoids.M/MC,morula/morula condensed;B,blastocyst.b,One-step protocol for the formation of human blastocyst-like structures. N2B27,serum-free medium;PALLY,PD0325901+A83-01+LPA+hLIF+Y-27632.c,Phase contrast image of human blastocyst-like structures formed after 96 hours on a nonadherent hydrogel microtiter plate. Each microtiter plate is 200 m in diameter. Scale bar: 400 m. d, Phase contrast images of representative human blastocyst-like structures taken from the microtiter plates. Scale bars: 200 and 100 m. e, Percentage of microtiter plates containing a human blastocyst-like structure for different naive PSC lines cultured under the PALLY conditions with optimized LPA concentration compared to a H9 control without the three inhibitors. Morphometric definition of blastocyst-like structures is indicated in Methods. n=3 microtiter plate arrays; mean±s.d.f,g,immunofluorescence of epiblast markers NANOGand OCT4,TE markers CDX2and GATA3and PrE markers SOX17and GATA4in human blastocyst-like structures.Scale bars,100 m. h, Absolute number of cells positive for OCT4, GATA3, and GATA4, and proportion of cells belonging to inpidual lineages, presented as a percentage of the total number of cells in blastocyst-like structures based on immunofluorescence. i, Representative immunofluorescence of tight junction molecule ZO-1, adherens junction molecule CDH1, and apical domain molecule aPKC in a representative human blastocyst-like structure, scale bar 50 m.

　　人早期胚胎模型可以较为准确地模拟早期人类胚胎发育以及人类胚胎植入子宫过程。

　　人类早期胚胎模型的建立，为将来研究人类生育，以及研究人类胚胎早期发育提供了重要研究基础。

　　乔森，助理研究员，以第一作者或通讯作者在Endocrinology，ACS Biomater Sci Eng，Front Neurosci，Front Molecular Biosciences发表多篇文章。